ABSTRACT Aim: The rapid increase in the use of food additives has paralleled the widespread consumption of ready-made and packaged foods. Additives such as sweeteners, colorants, preservatives, and thickeners have become unavoidable in daily life. In particular, sodium benzoate and potassium sorbate are widely used as powerful antimicrobial agents in the food and pharmaceutical industries. However, there needs to be more scientific data regarding the effects of their extensive use on human health. These uncertainties have highlighted the need for further investigation into the potentially harmful effects of sodium benzoate and potassium sorbate. This study aims to assess the cytotoxic effects of sodium benzoate and potassium sorbate on L929 mouse fibroblast and HOB human osteoblast cells based on their concentrations.